This is digital research data corresponding to a published manuscript in "Earthworms increase the potential for enzymatic bio-activation of biochars made from co-pyrolyzing animal manures and plastic wastes" J. Hazardous Materials 2021, Vol. 408, 124405. Dataset may be assessed via the included link at the Dryad data repository. We assessed the enzymatic activation of four different biochars produced from pyrolyzing swine manure and poultry litter, and by co-pyrolyzing these livestock residues with agricultural spent mulch plastic film wastes (plastichars). Enzymatic activation consisted of incubating biochars in soil inoculated with earthworms (Lumbricus terrestris), which acted as biological vectors to facilitate retention of extracellular enzymes onto biochar surface. The activity of carboxylesterase ‒a pesticide-detoxifying enzyme‒ was measured in non-bioturbed soils (reference), linings of the burrows created by earthworms, casts (feces) and biochar particles recovered from the soil. Methods are described in the manuscript https://doi.org/10.1016/j.jhazmat.2020.124405. Descriptions corresponding to each figure and table in the manuscript are placed on separate tabs to clarify abbreviations and summarize the data headings and units.