This entry contains all data and code required to reproduce the analysis of immune response data presented in the manuscript: Yeh, H.-Y., J. G. Frye, C. R. Jackson, Q. D. Read, J. E. Line, and A. Hinton. 2023. Use of automated capillary immunoassay for quantification of antibodies in chicken sera against recombinant Salmonella enterica serotype Heidelberg proteins. Journal of Microbiological Methods. https://doi.org/10.1016/j.mimet.2023.106757 Files included are: chicken_salmonella_immunoassay_analysis.Rmd: RMarkdown notebook with all analysis code and full supplementary results, figures, tables, and explanatory notes. The Excel file containing the data should be in the same dictionary as the .Rmd file when the notebook is rendered. chicken_salmonella_immunoassay_analysis.html: rendered output of notebook including full supplementary results, figures, and tables, and explanatory text. Chicken Sera FliD FimA.xlsx: Excel file with chicken immune response data in the form of chemiluminescence values (arbitrary units) that are a proxy for immunoglobulin concentration. Each row contains measurements from an individual bird, across two time points (bleedings); at each time point IgG chemiluminescence values in response to Salmonella FliD and FimA proteins are given, and IgM chemiluminescence as well. This results in a total of eight measurements per bird (2 bleedings x 2 types of immunoglobulin x 2 Salmonella proteins). The first ten rows are non-immunized control birds and the next ten rows are immunized birds. concentration_chemiluminescence.xlsx: Excel file with protein concentration and chemiluminescence data to verify relationship between them. There are three columns, one to identify the protein (FliD and FimA), one for known protein concentration, and one for chemiluminescence. This is used to verify that the chemiluminescence values are suitable proxies for protein concentration. This research is associated with USDA project 6040-32000-079-00D and falls under National Program NP108.

Data from a diet choice experiment on Mormon crickets Anabrus simplex (Orthoptera: Tettigoniidae) reared in the laboratory. Three groups were tested for immune response to inoculation with Beauveria bassiana fungus, an insect pathogen. One group had phenoloxidase (PO), prophenoloxidase (proPO), and total hemolymph protein assayed four times to create a time series from one day before to six days following treatment. A second group had PO, proPO and total protein assayed once on day four following treatment for comparison with those that were wounded multiple times, and a third group was assayed once on day one following treatment for comparison with those assayed on day four. Resources in this dataset: Resource Title: Contains descriptions of the data analyzed for Selective Protein Starvation by Mormon crickets Following Fungal Attack File Name: Readme.rtf Resource Description: Three related data sets are included with this text file. Resource Title: Protein and Carbohydrate Intake for Inoculated Mormon crickets and Controls File Name: InducedImmunityIdaho4dayIntakes.csv Resource Description: Dry mass consumption of P diet was averaged for days 1-3 or days 4-8, and multiplied by 0.42 to yield average daily protein consumption for the two time periods (in mg). The same was done to yield carbohydrate consumption. (c=control, i=inoculated, m=male, f=female) Resource Title: Phenoloxidase, proPhenoloxidase and total hemolymph protein for Mormon crickets relative to the day of fungal treatment (day 0) File Name: inducedImmunPOprotein.csv Resource Description: After eclosing to adults, male and female Mormon crickets were evenly separated into three experimental sets. Four inoculated females and four males (fdi and mdi, respectively) and four control females and four males (fdc and mdc, respectively) had hemolymph drawn on the day prior to the fungal treatments for baseline assays and then hemolymph drawn repeatedly every other day following treatment. In order to determine whether the injury to draw hemolymph affected the immunity titer, a second set of four inoculated females and four males (fai and mai, respectively) and four control females and four males (fac and mac, respectively) were given the fungal treatments as above and hemolymph was drawn once on day 4. A third set of four inoculated females and four males (fpi and mpi, respectively) and four control females and four males (fpc and mpc, respectively) were treated and hemolymph was drawn once on the day following treatment (day 1). Resource Title: Body mass and survivorship of Mormon crickets following fungal inoculation File Name: InducedImmunityIdahoMassSurvivorship.csv Resource Description: The twelve inoculated females (fdi, fai, fpi) and 12 males (mdi, mai, mpi) and 12 control females (fdc, fac, fpc) and 12 males (mdc, mac, mpc) were checked daily for mortality. Cadavers were placed in 100% humidity and checked for Beauveria sporulation (white spores) on the exoskeleton and membranes after 4 days. Mass on the day that the insect molted to adult, the day of inoculation, each day that hemolymph was drawn, and on the day the insect died were recorded.

This dataset is the raw Luminex antibody responses to six common waterborne pathogens reported in MFI (Median Fluorescence Intensity) units. This dataset is associated with the following publication: Augustine , S., T. Eason , K. Simmons, C. Curioso, S. Griffin , M. Ramudit, and T. Plunkett. Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens. Journal of Visualized Experiments. JoVE, Somerville, MA, USA, 115: e54415, (2016).