Laboratories and vivariums are maintained at ambient temperatures (Ta) of 20-24 ⁰C and it is widely accepted that mice maintained under these conditions are cold stressed. When mice are inactive and sleeping in the daytime, their zone of thermoneutrality associated with a basal metabolic rate is 30-32 ⁰C. If given a choice, mice will use thermoregulatory behavior to seek out thermoneutral temperatures during the daytime. The cold stress of a vivarium can be problematic to researchers requiring an animal model that is not stressed metabolically. However, it may not be practical or economically feasible to maintain an animal vivarium at thermoneutral temperatures. One problem with raising the Ta of a vivarium is that personnel wearing protective equipment will be subject to considerable heat stress. In this paper, we present plans for the construction and operation of a device that allows mice to utilize a refuge with a heated floor maintained at an approximate thermoneutral temperatures (30-32 ⁰C). The device is made of inexpensive, readily available materials and utilizes a disposable hand warmer (HotHands®) as a heat source. One hand warmer provides a thermoneutral environment for approximately 12 hours. This device is easily adapted to a standard mouse or rat cage and requires brief maintenance each day to change the heating pad. With this device in a standard cage, mice can select an environment associated with thermoneutral conditions during the daytime when inactive and sleeping. At night, the mice are more active, have a higher metabolic rate, and prefer cooler Ta’s. Egress from the warmed plate allows mice to seek cooler Ta’s at night. This dataset is associated with the following publication: Gordon, C., E. Puckett, E. Repasky, and A. Johnstone. A DEVICE THAT ALLOWS RODENTS TO BEHAVIORALLY THERMOREGULATE WHEN HOUSED IN VIVARIUMS. COMPARATIVE MEDICINE. American Association for Laboratory Animal Science, Memphis, TN, USA, 173-176, (2017).

This data shows the heart rate, heart rate variability and cardiac mechanical function of mice exposed to acrolein or ozone. This dataset is associated with the following publication: Kurhanewicz, N., R. McIntosh-Kastrinsky, H. Tong, A. Ledbetter, L. Walsh, A. Farraj, and M. Hazari. TRPA1 mediates changes in heart rate variability and cardiac mechanical function in mice exposed to acrolein. TOXICOLOGICAL AND APPLIED PHARMICOLOGY. Elsevier Science BV, Amsterdam, NETHERLANDS, 324: 51-60, (2017).