Data set one: on the population density of Meloidogyne hapla after inoculated on varying soil pH and soil texture during the study. Data set one was used for calculations and associated analyses of the parameters presented in Fig 1. Data set two: on the population density of Mesocriconema xenoplax after inoculated on varying soil pH and soil texture during the study, as well as associated plant metrics at harvest. Data set two was used for calculations and associated analyses of the parameters presented in Fig 2. Data set three: on the population density of Meloidogyne hapla and Mesocriconema xenoplax after inoculated on varying soil pH and soil texture during the study and associated plant metrics at harvest. Data set three was used for calculations and associated analyses of the parameters presented in Fig 3, and 4. Data set four: Root parameters measured using RhizoVision Explorer v2.0.3 on K-means clustered binary root mask images. Data set four was used for calculations and analysis of the parameters presented in Fig 5 and 6. Data set five: Measured soil parameters. Data set five was used for calculations and associated analyses in Table 1. *Note: Soil type is referred to as 'site' in all data sets. 'Site' was renamed to 'type' in the associated publication. Resources in this dataset: Resource Title: Data set 1: M. hapla File Name: M. hapla - soil texture and pH on M. hapla and M. xenoplax.csv Resource Title: Data set 2: M. xenoplax File Name: M. xenoplax - soil texture and pH on M. hapla and M. xenoplax.csv Resource Title: Data set 3: Nematode and host tomato metrics from harvest File Name: Harvest - soil texture and pH on M. hapla and M. xenoplax.csv Resource Title: Data set 4: Root measurements collected using RhizoVision Explorer v2.0.3 on tomato root images File Name: Root images - soil texture and pH on M. hapla and M. xenoplax.csv Resource Description: This data set was generated from RhizoVision Explorer v2.0.3, after preprocessing of root images. All root treatment data is the total of three seminal roots collected per treatment replicate; one from the top, middle, and bottom of the root mass. Resource Title: Data set 5: Soil parameter data File Name: Soil parameters - soil texture and pH on M. hapla and M. xenoplax.csv Resource Description: There are only two repeats (n=2) for each treatment combination (nematode x site x pH), one from each experimental repeat (August, October). Resource Title: Data Dictionary for all data sets File Name: Data Dictionary - soil texture and pH on M. hapla and M. xenoplax.csv Resource Description: Data Dictionary for all data sets for soil pH and texture effects on Meloidogyne hapla and Mesocriconema xenoplax, as well as host tomato metrics and soil metrics.

The USDA Nematode Collection is one of the largest and most valuable nematode collections in existence. It contains over 49,000 permanent slides and vials, with a total repository of nematode specimens reaching several million, including Cobb-Steiner, Thorne, and other valuable collections. Nematodes contained in this collection originate from world-wide sources. The USDA Nematode Collection Database contains over 38,000 species entries. A broad range of data is stored for each specimen, including species, host, origin, collector, date collected and date received. All records are searchable and available to the public through the online database. The physical collection is housed at the USDA Nematology Laboratory in Beltsville, MD. Specimens are available for loan to scientists who cannot personally visit the collection. Please see the Policy for Loaning USDANC Specimens for more information on this process. Scientists and other workers are always welcomed and encouraged to deposit material into the collection.