Unigene sequences were annotated by BlastX alignment to the non-redundant protein database (National Center for Biotechnology Information/GenBank) and the Aedes aegypti and Culex quinquefasciatus gene annotations (Vectorbase). This was done with a 1e-05 expectation value. Top hits are shown including accession numbers and description, if available. Unigene number and corresponding GenBank accession numbers are provided for all C. sonorensis genes. Both tables are modified from supplementary information tables at http://dx.doi.org/10.1371/journal.pone.0098123.s003 and numbered accordingly.

These files contain the geodatabases related to Brady's Geothermal Field. It includes all input and output files for the Geothermal Exploration Artificial Intelligence. Input and output files are sorted into three categories: raw data, pre-processed data, and analysis (post-processed data). In each of these categories there are six additional types of raster catalogs which are titled Radar, SWIR, Thermal, Geophysics, Geology, and Wells. These inputs and outputs were used with the Geothermal Exploration Artificial Intelligence to identify indicators of blind geothermal systems at the Brady Hot Springs Geothermal Site. The included zip file is a geodatabase to be used with ArcGIS and the tar file is an inclusive database that encompasses the inputs and outputs for the Brady Hot Springs Geothermal Site.

Deep sequencing technologies were used to construct the first adult female Culicoides sonorensis reference transcriptome. Genetic and genomic tools for Culicoides biting midges are lacking, despite the fact that they vector a large number of arboviruses and other pathogens impacting humans and domestic animals world-wide. Libraries of tissue-specific transcripts expressed in response to feeding and oral virus challenge in C. sonorensis have previously been reported, but extensive genome-wide expression profiling in the midge has not. Here, we successfully used deep sequencing technologies to construct the first adult female C. sonorensis reference transcriptome, and utilized genome-wide expression profiling to elucidate the genetic response to blood and sucrose feeding over time. The adult female midge unigene consists of 19,041 genes, of which less than 7% are differentially expressed during the course of a sucrose meal, while up to 52% of the genes respond significantly in blood-fed midges, indicating hematophagy induces complex physiological processes. Many genes that were differentially expressed during blood feeding were associated with digestion (e.g. proteases, lipases), hematophagy (e.g., salivary proteins), and vitellogenesis, revealing many major metabolic and biological factors underlying these critical processes. Additionally, key genes in the vitellogenesis pathway were identified, which provides the first glimpse into the molecular basis of anautogeny for C. sonorensis. This is the first extensive transcriptome for this genus, which will serve as a framework for future expression studies and in informing a reference genome assembly and annotation. Moreover, this study will serve as a foundation for subsequent studies of genome-wide expression analyses during early virus infection and dissecting the molecular mechanisms behind vector competence in midges.

HydroSHEDS (Hydrological data and maps based on SHuttle Elevation Derivatives at multiple Scales) provides hydrographic information in a consistent and comprehensive format for regional and global-scale applications. HydroSHEDS offers a suite of geo-referenced data sets in raster and vector format, including stream networks, watershed boundaries, drainage directions, and ancillary data layers such as flow accumulations, distances, and river topology information. Recently available data derived from HydroSHEDS include comprehensive layers of major basins and smaller sub-basins (~100-2,500 km2) across the globe. These data layers are available to support watershed analyses, hydrological modeling, and freshwater conservation planning at a quality, resolution, and extent that had previously been unachievable in many parts of the world. Data includes Void-Filled elevation, Hydrologically conditioned elevation, drainage directions, flow accumulation, river network, basin outlines, HydroBASINS License information: https://www.hydrosheds.org/page/license