Using the SHIME (an in vitro simulator of the human gut microbiome) we tracked the fate of the probiotic Lacticaseibacillus rhamnosus GG (LGG) over time and across colonic regions. Using fecal inoculum from three healthy human donors, reactors were established representing three colonic regions and both the luminal and mucosal microbiome in those regions. Community composition before, during, and after inoculation of the reactors with LGG as well as short chain fatty acid concentrations representing microbiome metabolic outputs. This dataset includes short-chain fatty acid concentrations and qPCR-based cell concentrations. Raw 16S rRNA amplicon sequencing of the V1-V2 regions can be found in the NCBI Sequence Read Archive associated with BioProject PRJNA893635: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA893635. Resources in this dataset: Resource Title: Short Chain Fatty Acid concentrations File Name: LGG_SCFA_data.csv Resource Title: LGG qPCR concentrations File Name: LGG_qPCR_data.csv Resource Title: Sample metadata File Name: LGG_16S_metadata.csv
OwnerUnited States Department of Agriculture - view all
Update frequencyunknown
Last updated10 months ago
Format
Overview16S rRNA sequence dataNP306probioticsshort chain fatty acids
Additional Information
KeyValue
dcat_modified2023-06-20
dcat_publisher_nameAgricultural Research Service
guid109638b5-21cb-44e4-8e87-e16da132bcda
language
harvest_object_idf7eb009b-5807-4ea1-9e88-b8d08b6709c0
harvest_source_id2c0b1e04-ba48-4488-9de5-0dab41f9913f
harvest_source_titleUSDA Open Data Catalog