Alternative Biomass Production Study for Resilient Economic Agricultural Practices in Morris, Minnesota The Tillage Study was established in 1997 to assess the effect of a variety of tillage intensities on soil C. The initial eight treatments included no-tillage, moldboard + disk tillage, chisel tillage, and fall and spring residue management, with or without strip-tillage and strip-tillage + subsoiling (Archer and Reicosky, 2009). In 2004, treatments were reduced to no-tillage, moldboard tillage, and fall and spring residue management without strip-tillage, but all had an early or late planting date. The last comprehensive set of soil samples were collected in 2006. In 2008, the strip-tilled subset of the Tillage Study plots were repurposed for the Alternative Biomass Production Systems study, which was designed to explore alternative strategies to support bioenergy including planting of cellulosic feedstock. The Alternative Biomass Production plots included perennials in an extended 6-year rotation, winter cereal rye cover crops in a corn-soybean rotation, and an alternative Sorghum-Sudan grass hybrid forage system, all of which have and will continue to be monitored for agronomic and soil properties.

Carbon Crops Study for Greenhouse gas Reduction through Agricultural Carbon Enhancement network and Resilient Economic Agricultural Practices in Morris, Minnesota The overall goal of the Carbon Crop study, established in 2000, was to assess strategies for increasing soil C sequestration including converting to no till systems and including perennial grasses (e.g., switchgrass and big bluestem) Overall, the goal of the study has remained constant, although individual treatments were changed after an incremental soil sampling, in response to new hypotheses and questions. Soil sampling is conducted as treatment changes are implemented. In 2012, two of the perennial grass systems (spring harvest of Switchgrass and Big Bluestem) were changed to corn/soybean rotations, beginning with a soybean entry point, to determine if the SOC accrued under the perennial system was lost by converting to a short annual rotation managed without tillage. The second change made was to compare the productivity between recent and traditional switchgrass cultivars. The final change was conversion of autumn harvest of Big Bluestem treatment replaced with an annual biomass crop – Sorghum-Sudan grass. Soil samples were taken to 1 m in 2000, 2006, 2011, and 2016. Nitrous oxide and carbon dioxide fluxes from the soil were measured from June 2009 through March 2012.