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Data from: Tephritid fruit fly gut bacterial population and community dynamics following adult emergence
OwnerUnited States Department of Agriculture - view all
Update frequencyunknown
Last updated10 months ago
Format
Overview

Data include microbial count data (CFUs), 16S-rRNA copy number data (qPCR), and microbial community (microbiome) data from the guts of the invasive tephritid fruit flies, melon fly (Zeugodacus cucurbitae) and medfly (Ceratitis capitata). Resources in this dataset: Resource Title: R code for dada2 processing and stacked bar charts of control microbiomes File Name: Control_Processing.zip Resource Description: Data showing performance of known controls (purchased from Zymo Research) using in-house DNA extraction and PCR methods for 16S-rRNA gene amplification and sequencing. Resource Title: Data processing of 16S amplicon data File Name: 16S SSU rRNA Microbiome Data Processing and Analysis.zip Resource Description: Raw data and accompanying R scripts for analysis of figure and generation of figures and tables. Data files include both amplicon sequence variant (ASV) count data matrix and accompanying ASV sequence files and taxonomies. Analysis and figure generation are made through independent R files. Resource Title: Data and analysis of fly culturable titers File Name: CFU titers.zip Resource Description: Colony forming units (CFUs) of fruit flies at different ages and the R code for figure generation and analysis. Resource Title: qPCR of 16S rRNA of Tephritid fruit flies at different ages File Name: 16S qPCR Titers.zip Resource Description: Raw data and R code of 16S rRNA copy numbers associated with medfly and melon fly gut tissues.

16S rRNA geneDipteraInsectsInvasive speciesNP304Tephritidaeinsect gut metagenomemicrobiome
Additional Information
KeyValue
dcat_modified2023-08-25
dcat_publisher_nameAgricultural Research Service
guid28ac86dd-d3ba-4ffc-9551-45d65a69ff58
language
harvest_object_id3e769927-ee30-47bc-bc89-8ea28978faf6
harvest_source_id2c0b1e04-ba48-4488-9de5-0dab41f9913f
harvest_source_titleUSDA Open Data Catalog
Files
  • ZIP
    R code for dada2 processing and stacked bar charts of control microbiomes
  • ZIP
    Data processing of 16S amplicon data
  • ZIP
    Data and analysis of fly culturable titers
  • ZIP
    qPCR of 16S rRNA of Tephritid fruit flies at different ages
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